Tyres: Inspect for any cuts or sections that are worn down from excessive braking. If you notice any damage, you should replace your tyres prior to the Tour de Brisbane. You don’t want to have your day ruined by an avoidable flat.
Wheels: Spin your wheels and check for any buckles. You will notice these if your rim wobbles towards one side of your frame. Its especially important to get these straightened prior to the Tour de Brisbane if you are using Rim Brakes. A buckle in the wheel will can mean that your braking will be uneven, and sometimes cause your wheel to skid. As well as this if you snap a spoke, you may not be able to finish the event.
Brakes: Check your brake pads and make sure that you are not close to the wear limit. If they are too worn, replace them. Also ensure that you are using the correct brake pads for the wheels you are using (IE Carbon Pads for Carbon Wheels)
Gears: Make sure that your drivetrain is shifting properly. Check your rear hanger alignment, and make sure that it is straight, and you do not tun the risk of throwing your derailleur into the spokes when shifting into the lower gears.
Nuts and bolts: Ensure all nuts and bolts are torqued to the correct setting. This is especially important if you have travelled to the race and had to dismantle your bike.
Cleats: Make sure that your cleats are not too worn. Try to do this a few weeks before the event, so that if you have to change them you allow yourself some time to make sure that they are positioned correctly.